Title
Utilization of marine industry waste derived collagen hydrolysate as peroxide inhibition agents in lipid-based food
Author
Jayashree Das, Pritha Dey, Tanuj Chakraborty, Kadharbasha Saleem, Rashmi Nagendra, Pradipta Banerjee
Year
2017
Journal
Journal of Food Processing and Preservation
Abstract
Marine industry waste is rich in the extracellular structural protein collagen, excised fragments of which are known to display numerous physiological activities. This study aims at screening waste derived collagen hydrolysates for their utility as peroxide inhibitors in lipid-based food and cytoprotective agents via cell culture. Collagen was isolated from Pacu and Rohu waste by acid dissolution and salt precipitation. The purified collagen samples were analyzed through electrophoresis, spectral, and elution pattern and confirmed to be of Type-I. The hydrolysates exhibited a molecular weight around 5 kDa and were found to be in random conformation. The hydrolysates substantially decreased peroxidation levels by 80–90% in linoleic acid model and were equally effective in market available products: cod liver oil and mustard oil. Cell culture assays showed that the hydrolysates were not toxic and capable of increasing cell survival rate by scavenging lipid peroxides generated in situ.
Instrument
J-715
Keywords
Circular dichroism, Secondary structure, Biochemistry, Food science