Title
Vitamin D prevents glycation of proteins: An In Vitro Study
Author
Sarah Iqbal, Md. Maroof Alam, Imrana Naseem
Year
2016
Journal
FEBS Letters
Abstract
Human serum albumin (HSA) is an important protein involved in the transport of hormones, fatty acids, drugs and other macromolecules. Under hyperglycemic conditions, this molecule undergoes irreversible modification that affects its structure and function. In the present study, we explored the effect of two forms of vitamin D, a nutraceutical, on glycation modification in HSA. The protein was incubated with a physiologically high concentration of glucose in the presence of vitamin D metabolites. After 21 days, samples were tested for secondary structural changes, side chain modification, and the presence of advanced glycation end products. Vitamin D metabolites could reduce glycation modification, albeit to a small extent. Interaction studies reveal that Vitamin D interaction with HSA can prevent protein glycation.
Instrument
J-???
Keywords
Circular dichroism, Secondary structure, Biochemistry