Title
Biocytin based pH-stimuli responsive supramolecular multivariant hydrogelator for potential applications
Author
Samala Murali Mohan Reddy, George Augustine, Niraikulam Ayyadurai, Ganesh Shanmugam
Year
2018
Journal
ACS Applied Biomaterials
Abstract
Self-assembly is one of the important and fruitful method to develop various biomaterials especially hydrogels from biologically important small molecules. Two such molecules are D-Biotin (B-vitamin), which plays several vital roles in biology and used in biomedical field, and Biocytin, which is used as intracellular marker and also to identify neurons. Despite the significance of these biomolecules, their usage in the form of hydrogel is very limited. Herein, for the first time, a pH stimuli-responsive supramolecular hydrogel based on Biocytin, an amidation product of D-Biotin and L-Lysine, has been developed to extend the use of D-Biotin, Biocytin and L-Lysine in a biomedical field. The newly developed hydrogelator such as Nα-(Fluorenylmethoxycarbonyl)-D-Biocytin) [FmB] exhibited pH-dependent hydrogelation and exhibited thixotropic and thermally reversible nature at physiological pH while it displayed super gelator nature at acidic pH. Electron microscopic analysis of FmB hydrogel indicated the formation of fibrous network structure. Various biophysical measurements including CD, FTIR, Fluorescence, and powder XRD demonstrated that intermolecular interactions such as aromatic and hydrogen bonding stabilize the fibrous structure of FmB. Importantly, FmB hydrogel found to be viable for the cells and displayed specific antibactericidal activity against E.Coil. Since making hydrogels directly with drugs or vitamins rather than inclusion in other hydrogel (matrix) may provide new biomaterials that can act as self-delivery systems, we believe that the newly developed multivariant Biocytin-based supramolecular hydrogel significantly advance the application of Biotin and Biocytin in various fields.
Full Article
Instrument
J-715
Keywords
Circular dichroism, Aggregation, Materials