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Home / Applications / Analysis of Aldehydes using Post-Column Derivatization by High Performance Liquid Chromatography

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Analysis of Aldehydes using Post-Column Derivatization by High Performance Liquid Chromatography

By Heather Haffner

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January 8, 2024

Introduction

It is becoming a big concern that aldehydes such as the formaldehyde and acetaldehyde act as an environmental pollutant that may contaminate the atmosphere, lakes, marshes, reservoirs, and rivers. Therefore, aldehydes are subject to various regulations like the Air Pollution Control Law, Water Supply Law, and Offensive Odor Control Law in Japan. As a method to measure aldehydes using HPLC, the pre-column derivatization method by 2,4- DNPH is well known, but this requires pretreatment such as sample collection, condensation and extraction before analysis. JASCO has introduced the analysis of formaldehyde and acetaldehyde by the post column fluorescence derivatization method using 1,3- Cyclohexanedione as a derivatizing reagent which doesn’t need such pretreatment.

The two above mentioned aldehydes and three others, propyl aldehyde, butyraldehyde, and valeraldehyde were analyzed simultaneously.

Jasco CO2 Delivery Pump: PU-2080-CO2

Experimental

Keywords

820023H

Results

In figure 1, the 1,3-Cyclohexanedione reaction during the post column derivatization method is shown and figure 2 illustrates flow diagram for the HPLC.

Figure 1. 1,3-Cyclohexanedione reaction formula of post column derivatization method

No.1 : Eluent
No.2 : Degasser (DG-2080-53)
No.3 : Pump for eluent (PU-2080)
No.4 : Autosampler (AS-2057)
No.5 : Column oven (CO-2060)
No.6 : Column (Shodex RSpak KC-811 6E)
No.7 : Reagent
No.8 : Pump for reagent (PU-2085)
No.9 : Reaction oven (RO-2061)
No.10: Reaction coil
No.11: Cooling coil
No.12: Fluorescence detector (FP-2020)
No.13: Backpressure coil

Figure 3. Chromatogram of 5 aldehydes and minimum detectable amount of each. 1: Formaldehyde (0.091 ng), 2: Acetaldehyde (0.105 ng), 3: Propylaldehyde (0.418 ng), 4: Butylaldehyde (0.593 ng), 5: Valeraldehyde (4.53 ng)
This document has been prepared based on information available at the time of publication and is subject to revision without notice. Although the contents are checked with the utmost care, we do not guarantee their accuracy or completeness. JASCO Corporation assumes no responsibility or liability for any loss or damage incurred as a result of the use of any information contained in this document. Copyright and other intellectual property rights in this document remain the property of JASCO Corporation. Please do not attempt to copy, modify, redistribute, or sell etc. in whole or in part without prior written permission.

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About the Author

JASCO Application Note

Analysis of Aldehydes using Post-Column Derivatization by High Performance Liquid Chromatography

Introduction

It is becoming a big concern that aldehydes such as the formaldehyde and acetaldehyde act as an environmental pollutant that may contaminate the atmosphere, lakes, marshes, reservoirs, and rivers. Therefore, aldehydes are subject to various regulations like the Air Pollution Control Law, Water Supply Law, and Offensive Odor Control Law in Japan. As a method to measure aldehydes using HPLC, the pre-column derivatization method by 2,4- DNPH is well known, but this requires pretreatment such as sample collection, condensation and extraction before analysis. JASCO has introduced the analysis of formaldehyde and acetaldehyde by the post column fluorescence derivatization method using 1,3- Cyclohexanedione as a derivatizing reagent which doesn’t need such pretreatment.

The two above mentioned aldehydes and three others, propyl aldehyde, butyraldehyde, and valeraldehyde were analyzed simultaneously.

Jasco CO2 Delivery Pump: PU-2080-CO2

Experimental

Results

In figure 1, the 1,3-Cyclohexanedione reaction during the post column derivatization method is shown and figure 2 illustrates flow diagram for the HPLC.

Figure 1. 1,3-Cyclohexanedione reaction formula of post column derivatization method

No.1 : Eluent
No.2 : Degasser (DG-2080-53)
No.3 : Pump for eluent (PU-2080)
No.4 : Autosampler (AS-2057)
No.5 : Column oven (CO-2060)
No.6 : Column (Shodex RSpak KC-811 6E)
No.7 : Reagent
No.8 : Pump for reagent (PU-2085)
No.9 : Reaction oven (RO-2061)
No.10: Reaction coil
No.11: Cooling coil
No.12: Fluorescence detector (FP-2020)
No.13: Backpressure coil

Figure 3. Chromatogram of 5 aldehydes and minimum detectable amount of each. 1: Formaldehyde (0.091 ng), 2: Acetaldehyde (0.105 ng), 3: Propylaldehyde (0.418 ng), 4: Butylaldehyde (0.593 ng), 5: Valeraldehyde (4.53 ng)

Keywords

820023H

This document has been prepared based on information available at the time of publication and is subject to revision without notice. Although the contents are checked with the utmost care, we do not guarantee their accuracy or completeness. JASCO Corporation assumes no responsibility or liability for any loss or damage incurred as a result of the use of any information contained in this document. Copyright and other intellectual property rights in this document remain the property of JASCO Corporation. Please do not attempt to copy, modify, redistribute, or sell etc. in whole or in part without prior written permission.
28600 Mary’s Court, Easton, MD 21601 USA • (800) 333-5272 • Fax: (410) 822-7526 • jascoinc.com/applications

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