Introduction
The use of commercial sweeteners in the manufacture of food and drinks is strictly regulated by agencies such as the FDA. This application note demonstrates a robust method for the analysis of saccharin (sodium salt), described in a Food Additive Test Method posted in an annotation of the Food Hygiene Law.
Experimental
Experiment
| Pump: | PU-2080 |
| Degasser: | DG-2080-53 |
| Column Oven: | CO-2060 |
| Autosampler: | AS-2057 |
| Detector: | UV-2075 |
Conditions
| Column: | CrestPak C18S (4.6 mmI.D. x 150 mmL, 5 µm) |
| Eluent: | 0.05 mol/L Phosphate buffer (pH 6.9)/Methanol (90/10) |
| Flow Rate: | 1.0 mL/min |
| Column Temp: | 40ºC |
| Wavelength: | 230 nm |
| Injection Volume: | 10 µL |
| Standard Sample: | Saccharin (sodium salt) 0.5 µg/mL in water |
Structural formula of saccharin (sodium salt) is shown in Fig. 1.
Keywords
Food additive, Sweetener, Saccharin sodium, C18 column, UV detection HPLC
Results
A chromatogram of a saccharin standard is shown in Fig. 2.
