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Home / Applications / Anion Analysis using Ion Chromatography with Chemical Suppression

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  • Technique

Anion Analysis using Ion Chromatography with Chemical Suppression

By Masao Bounashita

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October 9, 2024

Introduction

ICSpak is a suppressor technology for anion exchange chromatography developed by JASCO. The packing material has a sulfonic acid group chemically bonded to a styrene-divinylbenzene porous polymer and packed in a PEEK column. The suppressor is used in anion chromatography to decrease the background signal from the eluent and to change the sample to an ion-pair with high equivalent conductivity. This suppressor can be re-cycled using regeneration and washing steps. The more sensitive detection of nitrate and nitrite ions is made using a UV detector.

This report describes the analysis of anions using an inert anion exchange chromatography system with ICSpak suppressor.

Experimental

EquipmentConditions
Pump:PU-4080iColumn:Shodex IC SI-90 4E (4.6mmI.D. x 250mmL)
Pump Option:DG unitSuppressor:ICSpak (4.6mmI.D. x 100mmL)
Autosampler:AS-4050iEluent:1.8mM Na2CO3 + 1.7mM NaHCO3
Column Oven:CO-4060Flow rate:1.0mL/min
Detector:UV-4075 (Inert Cell)Column temp.:25ºC
 Shodex CD-5Wave length:215nm
  Injection volume:100 μL
  Standard Sample:Anion Mixture (Described below)
AnionsConc. [mg/L]
F-0.2
Cl-0.3
NO2-0.5
Br -1
NO3-1
PO43-1.5
SO42-1.5
flow path diagram of anion chromatography
Fig.1 The flow path diagram

Keywords

110020I, Anion Exchange Chromatography, Chemical Suppression, HPLC, conductivity detector, ion suppression.

Results

Fig.2 Chromatograms of anion standard solution mixture. 7-component anions.

Chromatograms of anion standard solution mixture using Shodex IC SI-90 4E and chemical suppressor ICSpak Top: UV detector, Bottom: Conductivity detector 1: F-, 2: Cl-, 3: NO2-, 4: Br-, 5: NO3-, 6: PO43-, SO42-
Figure 2. Chromatograms of anion standard solution mixture using Shodex IC SI-90 4E and chemical suppressor ICSpak Top: UV detector, Bottom: Conductivity detector 1: F-, 2: Cl-, 3: NO2-, 4: Br-, 5: NO3-, 6: PO43-, SO42-
Table 2 Detection limit for a range of anions.

The CO-4065 can be fitted with two optional valves. Analysis and regenerating/washing can be performed in parallel using the CO-4065 with two 2-position/6-port switching valves and two ICSpak suppressor columns with a multi-solvent regenerating/washing pump (e.g., the PU-4180 fitted with a low pressure gradient unit) as shown in the fig.3.

Equipment 
Pump:PU-4180
Pump Option:DG unit
 LPG unit
R-Pump Unit:R-PU-4080i
Autosampler:AS-4050i
Column Oven:CO-4065
Oven Option:2 way 6 port x 2
Detector:UV-4075 (Inert Cell)
 Shodex CD-5
Flow path of a system for parallel analysis and regenerating/washing
Figure 3 Flow path of a system for parallel analysis and regenerating/washing

 

This document has been prepared based on information available at the time of publication and is subject to revision without notice. Although the contents are checked with the utmost care, we do not guarantee their accuracy or completeness. JASCO Corporation assumes no responsibility or liability for any loss or damage incurred as a result of the use of any information contained in this document. Copyright and other intellectual property rights in this document remain the property of JASCO Corporation. Please do not attempt to copy, modify, redistribute, or sell etc. in whole or in part without prior written permission.

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About the Author

Masao Bounashita

JASCO Application Note

Anion Analysis using Ion Chromatography with Chemical Suppression

Introduction

ICSpak is a suppressor technology for anion exchange chromatography developed by JASCO. The packing material has a sulfonic acid group chemically bonded to a styrene-divinylbenzene porous polymer and packed in a PEEK column. The suppressor is used in anion chromatography to decrease the background signal from the eluent and to change the sample to an ion-pair with high equivalent conductivity. This suppressor can be re-cycled using regeneration and washing steps. The more sensitive detection of nitrate and nitrite ions is made using a UV detector.

This report describes the analysis of anions using an inert anion exchange chromatography system with ICSpak suppressor.

Experimental

EquipmentConditions
Pump:PU-4080iColumn:Shodex IC SI-90 4E (4.6mmI.D. x 250mmL)
Pump Option:DG unitSuppressor:ICSpak (4.6mmI.D. x 100mmL)
Autosampler:AS-4050iEluent:1.8mM Na2CO3 + 1.7mM NaHCO3
Column Oven:CO-4060Flow rate:1.0mL/min
Detector:UV-4075 (Inert Cell)Column temp.:25ºC
 Shodex CD-5Wave length:215nm
  Injection volume:100 μL
  Standard Sample:Anion Mixture (Described below)
AnionsConc. [mg/L]
F-0.2
Cl-0.3
NO2-0.5
Br -1
NO3-1
PO43-1.5
SO42-1.5
flow path diagram of anion chromatography
Fig.1 The flow path diagram

Keywords

110020I, Anion Exchange Chromatography, Chemical Suppression, HPLC, conductivity detector, ion suppression.

Results

Fig.2 Chromatograms of anion standard solution mixture. 7-component anions.

Chromatograms of anion standard solution mixture using Shodex IC SI-90 4E and chemical suppressor ICSpak Top: UV detector, Bottom: Conductivity detector 1: F-, 2: Cl-, 3: NO2-, 4: Br-, 5: NO3-, 6: PO43-, SO42-
Figure 2. Chromatograms of anion standard solution mixture using Shodex IC SI-90 4E and chemical suppressor ICSpak Top: UV detector, Bottom: Conductivity detector 1: F-, 2: Cl-, 3: NO2-, 4: Br-, 5: NO3-, 6: PO43-, SO42-
Table 2 Detection limit for a range of anions.

The CO-4065 can be fitted with two optional valves. Analysis and regenerating/washing can be performed in parallel using the CO-4065 with two 2-position/6-port switching valves and two ICSpak suppressor columns with a multi-solvent regenerating/washing pump (e.g., the PU-4180 fitted with a low pressure gradient unit) as shown in the fig.3.

Equipment 
Pump:PU-4180
Pump Option:DG unit
 LPG unit
R-Pump Unit:R-PU-4080i
Autosampler:AS-4050i
Column Oven:CO-4065
Oven Option:2 way 6 port x 2
Detector:UV-4075 (Inert Cell)
 Shodex CD-5
Flow path of a system for parallel analysis and regenerating/washing
Figure 3 Flow path of a system for parallel analysis and regenerating/washing

 

This document has been prepared based on information available at the time of publication and is subject to revision without notice. Although the contents are checked with the utmost care, we do not guarantee their accuracy or completeness. JASCO Corporation assumes no responsibility or liability for any loss or damage incurred as a result of the use of any information contained in this document. Copyright and other intellectual property rights in this document remain the property of JASCO Corporation. Please do not attempt to copy, modify, redistribute, or sell etc. in whole or in part without prior written permission.
28600 Mary’s Court, Easton, MD 21601 USA • (800) 333-5272 • Fax: (410) 822-7526 • jascoinc.com/applications

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