Introduction
ICSpak is a suppressor technology for anion exchange chromatography developed by JASCO. The packing material has a sulfonic acid group chemically bonded to a styrene-divinylbenzene porous polymer and packed in a PEEK column. The suppressor is used in anion chromatography to decrease the background signal from the eluent and to change the sample to an ion-pair with high equivalent conductivity. This suppressor can be re-cycled using regeneration and washing steps. The more sensitive detection of nitrate and nitrite ions is made using a UV detector.
This report describes the analysis of anions using an inert anion exchange chromatography system with ICSpak suppressor.
Experimental
| Equipment | Conditions | ||
|---|---|---|---|
| Pump: | PU-4080i | Column: | Shodex IC SI-90 4E (4.6mmI.D. x 250mmL) |
| Pump Option: | DG unit | Suppressor: | ICSpak (4.6mmI.D. x 100mmL) |
| Autosampler: | AS-4050i | Eluent: | 1.8mM Na2CO3 + 1.7mM NaHCO3 |
| Column Oven: | CO-4060 | Flow rate: | 1.0mL/min |
| Detector: | UV-4075 (Inert Cell) | Column temp.: | 25ºC |
| Shodex CD-5 | Wave length: | 215nm | |
| Injection volume: | 100 μL | ||
| Standard Sample: | Anion Mixture (Described below) |
| Anions | Conc. [mg/L] |
|---|---|
| F- | 0.2 |
| Cl- | 0.3 |
| NO2- | 0.5 |
| Br - | 1 |
| NO3- | 1 |
| PO43- | 1.5 |
| SO42- | 1.5 |
Keywords
Anion Exchange Chromatography, Chemical Suppression, HPLC, conductivity detector, ion suppression.
Results
Fig.2 Chromatograms of anion standard solution mixture. 7-component anions.
The CO-4065 can be fitted with two optional valves. Analysis and regenerating/washing can be performed in parallel using the CO-4065 with two 2-position/6-port switching valves and two ICSpak suppressor columns with a multi-solvent regenerating/washing pump (e.g., the PU-4180 fitted with a low pressure gradient unit) as shown in the fig.3.
| Equipment | |
|---|---|
| Pump: | PU-4180 |
| Pump Option: | DG unit |
| LPG unit | |
| R-Pump Unit: | R-PU-4080i |
| Autosampler: | AS-4050i |
| Column Oven: | CO-4065 |
| Oven Option: | 2 way 6 port x 2 |
| Detector: | UV-4075 (Inert Cell) |
| Shodex CD-5 |
System on page 1
System on page 2
