Introduction
In UHPLC one of the problems associated with taking advantage of the increased theoretical plates and higher resolution offered by longer columns packed with sub 2um phases is the extremely high back pressure at higher flow rates. This is made worse when water/methanol or water/ethanol mobile phases are used for gradient elution; even higher pressure will be caused by the increases in viscosity. To overcome these potential problems JASCO now offers the UHPLC system that can operate under the extremely high pressures up to 130 Mpa (19,500 psi). In this application note the JASCO X-LC-130 system was used for the fast analysis of alkylphenones using a sub 2um column with 150mm length at 1mL/min and with high column operating pressure (120 MPa).
Experimental
Equipment
| Pump: | X-LC 3185PU-130 |
| Degasser: | X-LC 3080DG |
| Column oven: | X-LC 3067CO |
| Autosampler: | X-LC 3159AS-130 |
| Detector: | X-LC 3110MD |
Conditions
| Column: | ZORBAX RRHD SB-C18 (2.1 mmID x 150 mmL, 1.8 µm) |
| Eluent: | Water/Acetonitrile (30/70) |
| Flow rate: | 1.0 mL/min |
| Column Temp.: | 40 ºC |
| Wavelength: | 254 nm |
| Injection Volume: | 1 µL |
| Column Pressure: | 120 MPa |
| Standard Sample: | Acetanilide, Acetophenone, Propiophenone, Butyrophenone Valerophenone, Hexanophenone, Heptanophenone, Octanophenone (2 µg/mL each) |
Keywords
UHPLC, alkylphenones, 130 MPa, 1.8 µm, C18 column, PDA detector
Results
A chromatogram and contour plot of the standard mixture of alkylphenones are shown in Fig. 1. The 8 alkylphenones were separated in under 2 minutes with complete baseline separation.
