Analysis of Sugar Alcohols by Semi-micro HPLC
August 18, 2022
Introduction
Semi-micro HPLC analysis offers a significant advantage compared with conventional separations in reducing separation time and cutting cost by reducing the consumption of mobile phase. However, HPLC systems designed for conventional separations often do not provide good enough peak shape or separation due to peak diffusion in the non-optimized flow path. When using a semi-micro column an optimized RHPLC system is recommended. This analysis report compares the measurement of sugar alcohols using a conventional refractive index detector with a detector optimized for semi-micro HPLC.
Experimental
| Equipment | Conditions | ||
|---|---|---|---|
| Pump: | PU-4185 | Column: | Inertsil Amide (2.1 mmI.D. x 250 mmL, 3 μm) |
| Pump Option: | Degasser unit | Eluent: | Water/Acetonitrile (30/70) |
| Autosampler: | AS-4150 | Flow rate: | 0.2 mL/min |
| Column Oven: | CO-4060 | Column temp.: | 30ºC |
| Detector: | RI-4035 | Injection volume: | 1 μL |
| Standard Sample: | Sugar alcohols mixture (5 mg/mL each) |
Keywords
Sugar Alcohols, reverse phase HPLC, refractive index, Glycerine, Erythritol, Xylitol, Solbitol, Inositol
Results
Fig. 1 shows chromatograms of sugar alcohols measured using a refractive index detector for conventional analysis (RI-4030) or for semi-micro analysis (RI-4035). The RI-4035 for semi-micro analysis obviously provides much-improved peak shape and resolution.
System Configuration
| P/N | Description |
|---|---|
| 7003-J014A | PU-4185 RHPLC Semi-micro Pump (Base Unit) |
| 7006-H003A | DG-4000-04 4-channel Degasser Unit, for Analytical |
| 7064-J002A | AS-4150 RHPLC Autosampler |
| 7021-J002A | CO-4060 Column Oven |
| 7031-J002A | RI-4035 Refractive Index Detector, for Semi-micro LC |
| 7058-J011A | BS-4000-1 Bottle stand |
| 6688-H564A | LC-Net CG cable (3x) |
| 7059-J012A | ChromNAV Ver.2 Chromatography Data System |
| 7001-H403A | RHPLC/UHPLC Start Up Kit for LC-4000 |
| 7001-H405A | Maintenance tool kit |
| Inertsil Amide (2.1 mmI.D. x 250 mmL, 3 μm) |
