Analysis of Sugar Alcohols by Semi-micro HPLC

Download PDF May 16, 2017

Introduction

Semi-micro HPLC analysis offers a significant advantage compared with conventional separations in reducing separation time and cutting cost by reducing the consumption of mobile phase. However, HPLC systems designed for conventional separations often do not provide good enough peak shape or separation due to peak diffusion in the non-optimized flow path. When using a semi-micro column an optimized RHPLC system is recommended. This analysis report compares the measurement of sugar alcohols using a conventional refractive index detector with a detector optimized for semi-micro HPLC.

Experimental

EquipmentConditions  
Pump:PU-4185Column:Inertsil Amide (2.1 mmI.D. x 250 mmL, 3 μm)
Pump Option:Degasser unitEluent:Water/Acetonitrile (30/70)
Autosampler:AS-4150Flow rate:0.2 mL/min
Column Oven:CO-4060Column temp.:30ºC
Detector:RI-4035Injection volume:1 μL
  Standard Sample:Sugar alcohols mixture (5 mg/mL each)

Keywords

Sugar Alcohols, reverse phase HPLC, refractive index, Glycerine, Erythritol, Xylitol, Solbitol, Inositol

Results

Fig. 1 shows chromatograms of sugar alcohols measured using a refractive index detector for conventional analysis (RI-4030) or for semi-micro analysis (RI-4035). The RI-4035 for semi-micro analysis obviously provides much improved peak shape and resolution.

Sugar alcohols
Figure 1. Chromatogram of Sugar alcohol samples 1: Glycerine, 2: Erythritol, 3: Xylitol, 4: Solbitol, 5: Inositol

System Configuration

P/NDescription
7003-J014APU-4185 RHPLC Semi-micro Pump (Base Unit)
7006-H003ADG-4000-04 4-channel Degasser Unit, for Analytical
7064-J002AAS-4150 RHPLC Autosampler
7021-J002ACO-4060 Column Oven
7031-J002ARI-4035 Refractive Index Detector, for Semi-micro LC
7058-J011ABS-4000-1 Bottle stand
6688-H564ALC-Net CG cable (3x)
7059-J012AChromNAV Ver.2 Chromatography Data System
7001-H403ARHPLC/UHPLC Start Up Kit for LC-4000
7001-H405AMaintenance tool kit
 Inertsil Amide (2.1 mmI.D. x 250 mmL, 3 μm)

About the Author

Masao Bounashita