Title
2′‐O,4′‐C‐Methylene‐bridged nucleic acids stabilize metal‐mediated base pairing in a DNA duplex
Author
Osamu Nakagawa, Akane Fujii, Yuki Kishimoto, Yusuke Nakatsuji, Natsumi Nozaki, Satoshi Obika
Year
2018
Journal
ChemBioChem
Abstract
The 2′‐O,4′‐C‐methylene‐bridged nucleic acid (2′,4′‐BNA/LNA), having an N‐type sugar conformation, effectively improves duplex‐forming ability. 2′,4′‐BNA/LNA is widely used to improve gene knockdown in nucleic acid‐based therapies and is used in gene diagnosis. Metal‐mediated base pairs (MMBPs) such as thymine(T)‐HgII‐T and cytosine(C)‐AgI‐C have been developed and used as attractive tools in DNA nanotechnology studies. This study aimed to investigate the application of 2′,4′‐BNA/LNA in the field of MMBP. 2′,4′‐BNA/LNA with 5‐methylcytosine stabilized MMBP of C with AgI ion. Moreover, the 2′,4′‐BNA/LNA sugar significantly improved the duplex‐forming ability of the DNA/DNA complex compared to that by the unmodified sugar. These results suggest that the sugar conformation is important for improving the stability of duplex‐containing MMBPs. Our results indicate that 2′,4′‐BNA/LNA can be applied not only to nucleic acid‐based therapies but also to MMBP technologies.
Instrument
J-720
Keywords
Circular dichroism, DNA structure, RNA structure, Chemical stability, Biochemistry