Title
A Dual Functional Fluorescent Sensor for Human Serum Albumin and Chitosan
Author
Jianzuo Li, Jindan Wu, Fengchao Cui, Xia Zhao, Yunqi Li, Yuan Lin, Ying Li, Jun Hu, Yong Ju
Year
2016
Journal
Sensors and Actuators B: Chemical
Abstract
A dual functional AIE active amphiphilic molecule, sodium N,N’-bis[4-(3-sulfonatopropoxyl)] salicylidene (BSPS), was designed and synthesized. Due to its amphiphilic nature, BSPS could spontaneously enter into human serum albumin (HSA) cavities primarily promoted by hydrophobic forces, which restricted the rotation of N-N single bond through rigidifying the conformation of BSPS, thus making BSPS display intense fluorescence with a broad working range (0–100 μg/mL) and a low detection limit (down to 6.11 μg/mL) for HSA. Meanwhile, BSPS could also aggregate on chitosan (CS) through electrostatic interactions between negative sulphate moieties in BSPS and positive amine groups in CS, consequently resulting in a sharp increase in fluorescence intensity, with a linear relationship from 0 to 10 μg/mL of CS and a detection limit of 0.58 μg/mL. Moreover, the outstanding biocompatibility of BSPS makes it an attractive multiple functional sensor candidate for detecting biomacromolecules in biosystem.
Instrument
J-820
Keywords
Circular dichroism, Secondary structure, Biochemistry, Materials