Title
Binding and/or hydrolysis of purine-based nucleotides is not required for IM30 ring formation
Author
Carmen Siebenaller, Lukas Schlosser, Benedikt Junglas, Martina Schmidt-Dengler, Dominik Jacob, Nadja Hellmann, Carsten Sachse, Mark Helm, Dirk Schneider
Year
2021
Journal
FEBS Letters
Abstract
IM30, the inner membrane-associated protein of 30 kDa, is conserved in cyanobacteria and chloroplasts. Although its exact physiological function is still mysterious, IM30 is clearly essential for thylakoid membrane biogenesis and/or dynamics. Recently, a cryptic IM30 GTPase activity has been reported, albeit thus far no physiological function has been attributed to this. Yet, it is still possible that GTP binding/hydrolysis affects formation of the prototypical large homo-oligomeric IM30 ring and rod structures. Here, we show that the Synechocystis sp. PCC 6803 IM30 protein in fact is an NTPase that hydrolyzes GTP and ATP, but not CTP or UTP, with about identical rates. While IM30 forms large oligomeric ring complexes, nucleotide binding and/or hydrolysis are clearly not required for ring formation.
Instrument
J-815
Keywords
IM30, GTP, ATP, CTP, UTP, nucleotide binding