Title
Bovine serum albumin interacts with silver nanoparticles with a “side-on” or “end on” conformation
Author
Nandita Dasgupta, Shivendu Ranjan, Dhabaleswar Patra, Priyanka Srivastava, Ashutosh Kumar, Chidambaram Ramalingam
Year
2016
Journal
Chemico-Biological Interactions
Abstract
As the nanoparticles (NPs) enter into the biological interface, they have to encounter immediate and first exposure to many proteins of different concentrations. The physicochemical interaction of NPs and proteins is greatly influenced not only by the number and type of proteins; but also the surface chemistry of NPs. To analyze the effects of NPs on proteins, the interaction between bovine serum albumin (BSA) and silver nanoparticles (AgNPs) at different concentrations were investigated. The interaction, BSA conformations, kinetics and adsorption were analyzed by UVeVisible spectrophotometer, dynamic light scattering (DLS), FT-IR spectroscopy and fluorescence quenching. DLS, FTIR and UVevisible spectrophotometric analysis confirms the interaction with minor alterations in size of the protein. Fluorescence quenching analysis confirms the side-on or end-on interaction of 1.5 molecules of BSA to AgNP. Further, pseudo-second order kinetics was determined with equilibrium contact-time of 30 min. The data of the present study determines the detailed evaluation of BSA adsorption on AgNP along with mechanism, kinetics and isotherm of the adsorption.
Instrument
J-715
Keywords
Circular dichroism, Secondary structure, Ligand binding, Nanostructures, Biochemistry, Materials