Title
Development and Validation of LC Method for the Simultaneous Estimation of Rosuvastatin Calcium and Olmesartan Medoxomil in Pharmaceutical Dosage Form
Author
Tripti Sharma, SI SUDAMC, Dannana Gowri Sankar
Year
2014
Journal
International Journal of ChemTech Research
Abstract
A simple, fast, and precise reverse phase, isocratic HPLC method was developed for the separation and quantification of Rosuvastatin calcium and Olme sartan medoxomil in bulk drug and pharmaceutical do sage form. The quantification was carried out using Symm etry C18 column (4.6mm×150mm, particle size 5.0μm) and mobile phase comprised of buffer: acetonitrile: tetrahydrofuran in the ratio of (71:25:4 v/v/v). T he flow rate was 1.5mL/min and the effluent was monitored at 248 nm. The retention time of Rosuvastatin calcium and Olmesartan medoxomil were 11.66±0.85 and 13.67±0.8 min respectively. The method was validated in terms of linearity, precision, accuracy, and specificity, li mit of detection and limit of quantitation. Lineari ty of Rosuvastatin calcium and Olmesartan medoxomil were in the range of 5-20 μg/mL and 20-80 μg/mL respectively. The proposed method is suitable for s imultaneous determination of Rosuvastatin calcium a nd Olmesartan medoxomil in pharmaceutical dosage form and bulk drug
Full Article
Instrument
HPLC
Keywords
HPLC, Rosuvastatin Calcium, Olmesartan Medoxomil, Pharmaceutical, Dosage, validation