Title
Encapsulation state of messenger RNA inside lipid nanoparticles
Author
Mark L. Brader, Sean J. Williams, Jessica M. Banks, Wong H. Hui, Z. Hong Zhou, and Lin Jin
Year
2021
Journal
Biophysical Journal
Abstract
Understanding the structure of messenger RNA (mRNA) lipid nanoparticles, and specifically the microenvironment of the mRNA molecules within these entities, is fundamental to advancing their biomedical potential. Here, we show that a permeating cationic dye, thionine, can serve as a cryogenic electron microscopy contrasting agent by binding selectively to encapsulated mRNA without disturbing lipid nanoparticle morphology. Cryo-electron microscopy images identify the mRNA location, revealing that mRNA may exist within solvent-filled cavities or may be substantially lipid associated.
Instrument
J-1500
Keywords
mRNA, lipids, nanoparticles, cationic dye, thionine