Extraction and Characterisation of Collagen from the Skin of Golden Carp (Probarbus Jullieni), a Processing By-Product

By Ali Muhammed Moula Ali, Soottawat Benjakul, Thaummanoon Prodpran, Hideki Kishimura

July 28, 2017

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Title

Extraction and Characterisation of Collagen from the Skin of Golden Carp (Probarbus Jullieni), a Processing By-Product

Author

Ali Muhammed Moula Ali, Soottawat Benjakul, Thaummanoon Prodpran, Hideki Kishimura

Year

2017

Journal

Waste and Biomass Valorization

Abstract

Fish skin is a by-product generated during fish processing. Nowadays the farmed fish, including golden carp, has gained increasing interest from consumers. As a consequence, a huge amount of skin is generated. Those skins can serve as an important source of collagen, which can be used for several applications in food, pharmaceutical and biomedical industries. Additionally, collagen with high market value can increase the revenue for the farmer or fish processor. This study aimed to extract and characterise collagen from skin of golden carp. Acid solubilised collagen (ASC) and pepsin solubilised collagen (PSC) were isolated from the skin of golden carp (Probarbus jullieni). Obtained collagens were subjected to SDS–PAGE and amino acid analysis. Structural integrity was determined via FTIR and CD spectra. DSC and solubility were also examined. Both ASC and PSC were characterised as type I collagen. Imino acid contents of ASC and PSC were 197 and 199 residues/1000 residues, respectively. Glycine constituted approximately 1/3 of total amino acid residues. No cysteine was present, indicating the absence of disulphide bonds. FTIR and CD spectra were almost similar between ASC and PSC. Thus pepsin hydrolysis had no marked effect on triple helical structure. ASC and PSC showed higher Tmax values 36.28 and 37.87 °C, respectively, as compared to those from temperate and cold water fish collagens. The maximum solubility for both collagens was found at pH 3. Skin of golden carp, a by-product from fish processing, could therefore serve as an alternative source of high quality collagen. Subsequently the economic value of fish skin could be maximised and disposal problem could be reduced.

Full Article

http://link.springer.com/article/10.1007/s12649-017-9841-0

Instrument

J-810

Keywords

Circular dichroism, Secondary structure, Biochemistry