Impact of mesoporous silica surface functionalization on human serum albumin interaction, cytotoxicity and antibacterial activity
Malgorzata Ferenc, Nadia Katir, Katarzyna Milowska, Mosto Bousmina, Younes Brahmi, Aleksandra Felczak, Katarzyna Lisowska, Maria Bryszewska, Abdelkrim El Kadib
Microporous and Mesoporous Materials
Aiming to broaden our understanding on the interplay of silica nanomaterials with living biocells, we designed herein various surface-functionalized mesoporous SBA-15-type silicates, including native one SBA-OH, aminated SBA-NH2, thiolated SBA-SH and carboxy-terminated SBA-COOH. These high-surface area materials are then subjected to interactions with human serum albumin (HSA), in order to reproduce the real picture of what occurs within the body upon delivering active drugs. First, we examined circular dichroism measurements, fluorescence quenching, HSA adsorption and hemolysis in the presence and absence of HSA. Subsequently, we studied the toxicity of these materials toward eukaryotic and prokaryotic cells and the effect of the silica surface on the antibacterial activity. Irrespective to the functional groups, silica interacts with HSA resulting in the suppression of hemolysis. In terms of antibacterial activity, the pivotal parameter was found to be the amorphous state of the silica carrier rather than the nature of the functional groups. In the whole, this informative work provides an overall understanding of the role of the silica surface for bio-cells interaction and can serve as a guideline for the rational-design of blood-compatible nanomaterials.
Circular dichroism, Secondary structure, Nanostructures, Materials, Biochemistry