Isolation and characterization of wound-induced compounds from the leaves of Citrus hassaku

July 28, 2017

Title

Isolation and characterization of wound-induced compounds from the leaves of Citrus hassaku

Author

Tomonori Asai, Tetsuya Matsukawa, Atsushi Ishihara, Shin'ichiro Kajiyama

Year

2016

Journal

Journal of Bioscience and Bioengineering

Abstract

Citrus plants are world widely cultivated as horticultural tree crops, and nowadays their pharmacological activities have been well studied. Since research of defense responses in citrus plants have been mainly focused on the post-harvested fruits because of their commercial importance, defense mechanisms during their developmental stages have not been well understood. In the present study, two wound-induced compounds were isolated from leaves of Citrus hassaku, and their structures were elucidated by high-resolution electron spray ionization mass spectra (HRESIMS) and nuclear magnetic resonance (NMR) analyses. One of these compounds was identified as a known flavanone, hesperetin. The other was characterized as a novel furofuran lignan, and was named ‘biscitrusnin-A’. Their antimicrobial activities were also evaluated.

Instrument

J-820

Keywords

Circular dichroism, Agricultural and environmental