Title
Plant based production of myoglobin – a novel source of the muscle heme-protein
Author
Magnus L. R. Carlsson, Selvaraju Kanagarajan, Leif Bülow, Li-Hua Zhu
Year
2020
Journal
Scientific Reports
Abstract
Myoglobin is a heme-protein in the muscle of vertebrates with important functions in the oxygenation of tissues and as a regulator in nitric oxide signaling. Myoglobin from many species is also an important nutritional source of bioavailable iron. In this study, we have successfully produced human myoglobin in the leaves of Nicotiana benthamiana by transient expression using a viral vector delivered by Agrobacterium tumefaciens. Analyses confirmed that heme was incorporated and the protein was functional, with observed properties consistent with those of native myoglobins. A relatively high degree of purity could be achieved with low cost methods. The results show the high potential of plants as a production platform for heme proteins, a group of proteins of interest for iron nutrition applications and possible future pharmaceutical development.
Full Article
Instrument
J-815
Keywords
Circular dichroism, Secondary structure, Biochemistry