Selective recognition of human telomeric G-quadruplex with designed peptide via hydrogen bonding followed by base stacking interactions

By Shikhar Tyagi, Sarika Saxena, Nikita Kundu, Taniya Sharma, Amlan Chakraborty, Sarvpreet Kaur, Daisuke Miyoshi, Jadala Shankaraswamy

March 24, 2020

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Title

Selective recognition of human telomeric G-quadruplex with designed peptide via hydrogen bonding followed by base stacking interactions

Author

Shikhar Tyagi, Sarika Saxena, Nikita Kundu, Taniya Sharma, Amlan Chakraborty, Sarvpreet Kaur, Daisuke Miyoshi, Jadala Shankaraswamy

Year

2019

Journal

RSC Advances

Abstract

We described a novel synthetic peptide in which a glutamine residue binds through hydrogen bonding to a guanine-base and a trytophan residue intercalates with K+ resulting in stabilization of a human telomeric G-quadruplex with high selectivity over its complementary c-rich strand and a double-stranded DNA and its complementary C-rich strand. This peptide offers great potential for cancer treatment by inhibiting the telomere extension by telomerase.

Full Article

https://pubs.rsc.org/en/content/articlehtml/2019/ra/c9ra08761c

Instrument

J-715, FP-8300

Keywords

Circular dichroism, Fluorescence, Ligand binding, G-quadruplex structure, DNA structure, Biochemistry