Title
Spectroscopic investigation of the effects of aqueous-phase prepared CdTe quantum dots on protein hemoglobin at the molecular level
Author
Dandan Guo, Rutao Liu
Year
2017
Journal
Journal of Biochemical and Molecular Toxicology
Abstract
3-Mercaptopropionic Acid-modified CdTe quantum dots (QDs) were synthesized and characterized by infrared, fluorescence, and ultraviolet–visible absorption spectra and Nano-ZetaSizer measurements. Then the interaction between QDs and hemoglobin was studied to investigate the effects of QDs on the structure and function of hemoglobin by using a variety of spectroscopy methods and isothermal titration calorimetry. The results showed van der Waals forces and hydrogen bonding predominantly played major roles in the binding. The intrinsic fluorescence of hemoglobin was quenched with changes to the microenvironment of tyrosine and tryptophan residues and complex conformational changes of hemoglobin were induced with the loosening and unfolding skeleton. However, the heme in hemoglobin was still stable, indicating that the main physiological function of hemoglobin might not be significantly inhibited. This study will provide a new strategy to study the biological toxicity of QDs at the molecular level.
Instrument
J-810
Keywords
Circular dichroism, Secondary structure,Nanostructures, Ligand binding, Materials, Biochemistry