Biophysical characterization of HRC peptide analogs interaction with heptad repeat regions of the SARS-coronavirus Spike fusion protein core

2006 / Journal of Structural Biology

The Spike (S) protein of SARS-coronavirus (SARS-CoV) mediates viral entry into host cells. It contains two heptad repeat regions, denoted HRN and HRC. We …

Leah Pandiscia, PhD

Differential domain structure stability of the severe acute respiratory syndrome coronavirus papain-like protease

2012 / Archives of Biochemistry and Biophysics

Papain-like protease (PLpro) from severe acute respiratory syndrome (SARS) coronavirus is one of the two proteases involved in the proteolytic processing of the virion polyproteins. In …

Leah Pandiscia, PhD

Reversible Unfolding of the Severe Acute Respiratory Syndrome Coronavirus Main Protease in Guanidinium Chloride

2007 / Biophysical Journal

Chemical denaturant sensitivity of the dimeric main protease from severe acute respiratory syndrome (SARS) coronavirus to guanidinium chloride was examined in terms of fluorescence spectroscopy, circular …

Leah Pandiscia, PhD

Construct design, biophysical, and biochemical characterization of the fusion core from mouse hepatitis virus (a coronavirus) spike protein

2004 / Protein Expression and Purification

Membrane fusion between virus and host cells is the key step for enveloped virus entry and is mediated by the viral envelope fusion …

Leah Pandiscia, PhD

Mutation of Gly-11 on the Dimer Interface Results in the Complete Crystallographic Dimer Dissociation of Severe Acute Respiratory Syndrome Coronavirus 3C-like Protease

2007 / Journal of Biological Chemistry

SARS-CoV 3C-like protease (3CLpro) is an attractive target for anti-severe acute respiratory syndrome (SARS) drug discovery, and its dimerization has …

Leah Pandiscia, PhD

Characterization of the Heptad Repeat Regions, HR1 and HR2, and Design of a Fusion Core Structure Model of the Spike Protein from Severe Acute Respiratory Syndrome (SARS) Coronavirus†

2004 / Biochemistry

Severe acute respiratory syndrome coronavirus (SARS-CoV) is a newly emergent virus responsible for a worldwide epidemic in 2003. The coronavirus …

Leah Pandiscia, PhD

Role of phosphorylation clusters in the biology of the coronavirus infectious bronchitis virus nucleocapsid protein

2008 / Virology

The coronavirus infectious bronchitis virus (IBV) nucleocapsid (N) protein is an RNA binding protein which is phosphorylated at two conserved clusters. Kinetic analysis of RNA binding indicated that the C-terminal phosphorylation cluster …

Leah Pandiscia, PhD

Self-Assembly of Exendin-4-Derived Dual Peptide Agonists is Mediated by Acylation and Correlated to the Length of Conjugated Fatty Acyl Chains

2020 / molecular pharmaceutics

Dual glucagon-like peptide-1/glucagon receptor agonists have emerged as promising candidates for the treatment of diabetes and obesity. Issues of degradation …

Leah Pandiscia, PhD

Phosphorylation of the arginine/serine dipeptide‐rich motif of the severe acute respiratory syndrome coronavirus nucleocapsid protein modulates its multimerization, translation inhibitory activity and cellular localization

2008 / The FEBS Journal

Coronavirus nucleocapsid protein is abundant in infected cells and participates in viral RNA replication and transcription. The central domain of …

Leah Pandiscia, PhD

Following the rule: formation of the 6-helix bundle of the fusion core from severe acute respiratory syndrome coronavirus spike protein and identification of potent peptide inhibitors

2004 / Biochemical and Biophysical Research Communications

Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) is a newly identified member of Family Coronaviridae. Coronavirus envelope spike protein S is a class I viral fusion …

Leah Pandiscia, PhD