Title
The effect of manganese(II) on DNA structure: electronic and vibrational circular dichroism studies
Author
A. M. Polyanichko, V. V. Andrushchenko, E. V. Chikhirzhina, V. I. Vorob'ev, H. Wieser
Year
2004
Journal
Nucleic Acids Research
Abstract
The interaction of DNA with Mn2+ was studied in absorbance and optical activity in the electronic and vibrational regions. Based on the data, several stages of the interaction were identi®ed. Conformational transition towards the C-form of DNA was observed in solution at the molar ratio Mn2+/DNA-phosphates between 0.1 and 1.5. The exact ratio depended on the ionic strength and increased with increasing NaCl concentration. Although manganese interacted with the phosphates and bases of DNA at higher metal concentrations, it is unlikely that direct chelation occurred. A model for the interaction between manganese ions and DNA mediated by water is suggested destabilizing the double helix and partially breaking the hydrogen bonds between the base pairs. At high Mn2+ concentrations DNA aggregation was observed.
Full Article
Instrument
J-715
Keywords
Circular dichroism, DNA structure, Ligand binding, Biochemistry