Fluorescence Quantum Yield
Fluorescence Quantum Yield measurements provide information about the efficiency of a molecule’s fluorescence and is defined as the ratio of the number of photons absorbed to the number of photons emitted by a sample. Different molecular and environmental conditions not only effect whether a molecule will fluoresce or not, but can also determine the intensity of the emitted fluorescence radiation. Fluorescence quantum yield is measured using an integrating sphere to ensure that all sample fluorescence is collected.
ISF-134 | 60 mm Integrating Sphere
For quantum efficiency and color evaluation measurements of opaque solid or powder samples.
ILF-135 | 120 mm Integrating Sphere
For quantum efficiency measurements of liquids or thin membrane samples on a transparent substrate as well as opaque solid or powder samples.
ILFC-147 | Liquid N2 Cooled 120 mm Integrating Sphere
Cools samples with liquid nitrogen for fluorescence quantum yield and phosphorescence lifetime measurements. It can also be used at ambient temperatures without liquid nitrogen.
Specifications
| Model | IFS-134 | ILF-135 | ILFC-147 |
|---|---|---|---|
| Inner Diameter | 60 mm | 120 mm | 120 mm |
| Min. Sample Size | 20 (H) x 20 (W) x 0.5 (T) mm | 20 (H) x 10 (W) x 0.5 (T) mm | 20 (H) x 10 (W) x 0.5 (T) mm |
| Max. Sample Size | 60 (H) x 50 (W) x 25 (T) mm | 30 (H) x 20 (W) x 6 (T) mm | 30 (H) x 20 (W) x 6 (T) mm |
| Cells | PSH-004 (Std), PSH-002, PSH-003 PSH-005 (optional) | 1, 2 mm Liquid cell, 3 mm powder cell, 10 mm rectangular cell, KBr plate sample holder | 1, 2 mm Liquid cell, 3 mm powder cell, 10 mm rectangular cell, KBr plate sample holder, LPH-140, PPH-150, CPH-160 |
| Optional Spectral Correction Accessories | ESC-842, ESC 843 | ||
Cells and Cell Holders for Integrating Spheres
1 mm Liquid Cell
1 x 10 mm path length, 200 μL sample volume
2 mm Liquid Cell
2 x 10 mm path length, 400 μL sample volume
3 mm Powder Cell
19 (H) x 10 (W) x 3 (T) mm
10 mm Rectangular Cell Holder
Used to mount a 10 x 10 mm rectangular cell inside the ILF-835/ILFC-847 integrating spheres.
KBr Plate Sample Holder
Used to sandwich a powder sample between two KBr plates (5 × 5 × 1 mm). The KBr sample holder can also be used for micro FTIR measurements.