FP-8050 Series Instrument Performance

FP-8050 Series Instrument Performance

Outstanding Sensitivity and Linear Dynamic Range

The high-throughput optical system and low noise signal processing of the FP-8050 series results in an extremely high signal-to-noise (S/N) performance up to 8,500:1* (RMS). In addition, the FP-8050 series has been enhanced to provide up to 7 orders of magnitude of linear dynamic range. As shown in the calibration curve for fluorescein, there is excellent linearity even for samples at very low concentrations.

Calibration curve for fluorescein
(Auto Sensitivity Control System (SCS) ON)
Fluorescein spectra for a range of concentrations








Higher-Order Light Cut-Off Filters

3D Spectra of Fluorescent Orange Color Plate
3D Spectra of Fluorescent Orange Color Plate

In order to remove peaks originating from higher-order diffracted light, cut-off filters appropriate to the measurement wavelength should be used. The FP-8050 Series (excl. FP-8250) include cut-off filters that are matched to the wavelength range and switched automatically .

Accurate Spectral Correction

Spectral correction should be made to any fluorometer that is used for defining accurate spectral data. All models in the FP-8050 Series can be spectrally corrected with a simple procedure that uses a range of calibration standards, including broad-spectrum standard light sources and Rhodamine B.

Auto Adjustment of Detector Gain and Sensitivity

The FP-8050 Series includes both Auto-Gain and Auto-Sensitivity Control System (SCS), which automatically adjust the detector gain and sensitivity for optimum measurement. Auto-Gain automatically adjusts the gain of the signal from the detector so that the S/N is optimized throughout the entire scan range for spectral measurement, and weak fluorescence spectral peak shapes can be observed against scattered light with a high S/N. The Auto-SCS allows the user to create calibration curves for a wide concentration range without having to manually change the instrument sensitivity settings.

Fluorescence spectra of quinine sulfate solution

*Typical S/N for FP-8500 for water Raman, using on peak baseline noise.

Automatic Band-Pass Filter for Higher-Order Diffraction

The automatic band-pass filters remove peaks originating from higher-order diffracted light fr artifact-free spectral acquisition and analysis. The band-pass filters can also be used for spectral corrections, 3D measurements and quantum yield determination.

Automatic Accessory Recognition

Accessories are automatically recognized when installed in the instrument. Spectra Manager™ logs the accessory name and serial number; this information is saved in the data file for a complete record of the measurement.

Long-Life Light Source

Continuous output xenon arc lamps offer the greatest sensitivity. For the longest and highest performance, the FP-8050 series now includes a newly designed Xe arc lamp with prolonged lifetime. This new light source can be used up to 3x longer than previous lamps (maximum operating time: approximately 3000 hours), with long-term stable measurements, reduced maintenance and lower running costs.

Daily Monitoring of Instrument Performance

Validation program
The Validation program includes a full suite of tests to validate the total instrument performance. When executed, simple prompts guide the user on how to perform the tests. All instruments in the FP-8050 series include a mercury lamp as standard for wavelength calibration.

Daily Check Program

Daily Check program
The Daily Check is a less comprehensive performance check can be made at any time for continuous performance monitoring. After starting the Daily Check program, a timer is activated, and sample measurement is executed automatically after a predetermined stabilization time. The results are collated over time and a batch display lets the user review the ongoing performance of their instrument.

Instrument Usage Records

Instrument Activity Log

The instrument activity log is a useful alternative to the paper log books found in multi-user facilities. A record is maintained of user, duration of operation and lamp usage.