FP-8000 Series Instrument Performance

Highest Sensitivity

The high throughput optical system and low noise signal processing of the FP-8000 Series provides users with a high S/N (signal-to-noise) performance of up to 5,000:1 (RMS). The advanced A/D converter enables rapid sampling and the high speed signal processing system that immediately converts the fluorescence signal from the detector to a digital signal without introducing any additional noise.

Wide Dynamic Range

A wide dynamic range for luminescence measurements is obtained by using the Auto-Gain and Auto-SCS features, automatically adjusting the detector gain and sensitivity for optimum measurements.

Auto-Gain

Auto-Gain automatically adjusts the gain of a signal from the detector, so that the S/N is optimized throughout the entire scan range for spectral or time course measurements. Since quantum yield measurements can produce peaks that vary greatly in fluorescence intensity, the auto-gain function assures the accuracy of the measurement.

Auto-SCS

The Auto Sensitivity Control System (SCS) allows users to create calibration curves for a wide concentration range without having to manually change the instrument Sensitivity settings. Auto-SCS can obtain measurements of sub-picomolar to micromolar concentrations for fixed wavelength measurements and quantitative analyses.

Spectra of Fluorescein Solutions and Calibration Curve of Fluorescein Solutions
Fluorescein Solutions: Spectra (L) and Calibration Curve (R)

Automatic Band-Pass Filter for Higher-Order Diffraction

The new automatic band-pass filters remove peaks originating from higher-order diffracted light to provide simple and reliable spectral acquisition and analysis. The band-pass filters can also be used for spectral corrections, 3D measurements, and quantum yield determination.

3D Spectra of Fluorescent Orange Color Plate
3D Spectra of Fluorescent Orange Color Plate

Spectral Correction

JASCO has developed a spectral correction system to provide greater accuracy covering a larger wavelength range. While traditionally Rhodamine B was used for spectral correction of the excitation spectra, the use of calibrated deuterium and tungsten halogen light sources and calibrated detector now provide corrected data which can be directly observed from the UV to NIR spectral regions.

Automatic Accessory Recognition

When an accessory is attached to the instrument sample compartment, Spectra Manager™ automatically recognizes the installed accessory. Previously used instrument parameters associated with the accessory are recalled and information such as the accessory name and serial number are logged with the measurement file information. The IQ start system in conjunction with IQ Accessory recognition allows rapid access to pre-defined analysis methods.