FP-8050 Series Overview
JASCO’s FP-8050 Series fluorescence spectrofluorometers scale from routine benchtop analysis to diverse research applications. Four models (FP-8250, FP-8350, FP-8550, and FP-8650 NIR) share a common optical platform and Spectra Manager™ software, so labs can choose an instrument by performance and flexibility needs. Intuitive software and a shared accessory ecosystem keep the entry-level FP-8250 approachable for teaching and routine QA/QC labs. FP-8550 and FP-8650 offer advanced experimental capabilities and a wide range of accessories including integrating spheres and fiber optics.
Compare Spectrofluorometer Models
FP-8250 Spectrofluorometer
Simple and sensitive system which readily accommodates routine measurements and accessories, such as spectral scanning, quantitation, and temperature control.
- Wavelength range: 200-750 nm
- A compact, entry-level fluorometer built for routine QA/QC, teaching labs, and temperature-dependent quantitation
- Compatible with autosamplers and sippers
FP-8350 Spectrofluorometer
A powerful combination of performance, sensitivity and flexibility for biological, environmental and materials analysis.
- Wavelength range: 200-750 nm (Optional: 200-900 nm)
- Automatic higher order diffraction light cut-off filters
- A spectral bandwidth 1.0 nm optical design for high-resolution biological and biochemical research
- Compact for routine use in research and QA/QC laboratories.
FP-8550 Spectrofluorometer
Sophisticated optical system offering the ultimate in sensitivity, spectral accuracy, and flexibility for the most challenging materials and biological samples.
- Wavelength range: 200-850 nm
- Flagship steady-state model, rated for S/N greater than 8,500:1 (RMS, water Raman)
- Dynamic range to seven orders of magnitude and scan speeds up to 60,000 nm/min
- Compatible with integrating spheres for absolute quantum yield
FP-8650 NIR Spectrofluorometer
Uniquely optimized for NIR applications with extended wavelength measurement to 1010 nm.
- Excitation Range: 200-850 nm
- Emission Range: 200-980 nm (Optional: 200-1010 nm)
- Purpose-built for near-infrared fluorophores and materials-characterization work.
- Scan speeds up to 120,000 nm/min
When to choose the FP-8650 NIR: work that extends into the near-infrared range, such as carbon nanotube characterization or NIR dyes, benefits from the FP-8650’s extended emission detection to 1,010 nm.
Applications by Model
| Application Area | Best-Fit Model(s) |
|---|---|
| Routine QA/QC, simple quantitation and teaching | FP-8250 |
| Biological and biochemical research (proteins, enzymes, cell biology) | FP-8350, FP-8550 |
| Environmental and water quality (DOM, EEM scanning), biopharma characterization | FP-8350, FP-8550 |
| Materials science (quantum dots, thin films, LEDs, solar cells) | FP-8550, FP-8650 |
| Near-infrared applications (carbon nanotubes, biological tissue, NIR dyes) | FP-8650 |
| High-throughput and microplate workflows | FP-8250, FP-8350, FP-8550, FP-8650 (with microplate accessory) |
Advanced Capabilities
Beyond routine fluorescence spectra, the FP-8050 fluorometers support a full range of advanced measurement modes through optional modules:
- Anisotropy and polarization: for protein-binding, membrane fluidity, and molecular-rotation studies.
- Kinetics: real-time reaction and enzyme-kinetics monitoring.
- 3D excitation-emission matrix (EEM) scanning: full spectral mapping for water-quality, DOM, and biopharma characterization.
- Quantum yield determination: absolute quantum yield measurement with the appropriate accessory module.
Our FP-8550 and FP-8650 fluorometers offer the broadest compatibility across these advanced modes, supporting all accessories for demanding physics and materials-research applications.
Data integrity and compliance: Spectra Manager™ software supports secure user management, audit trails, electronic signatures, and controlled data export to help meet 21 CFR Part 11 and similar requirements.
Buyer’s Guide: Choosing Your Light Source
Which light source is right for your samples?
A continuous xenon source is highly stable giving a steady light output. This is essential for keeping fluorescence intensity consistent between samples for time resolved experiments or continuous FRET analysis. A pulsed xenon source is beneficial for reducing photodegredation but is more unstable and inconsistent in its light output making it unsuitable for melting, kinetics or experiments where high S/N is needed.
Accessories & High-Throughput Options
Accessories extend the FP-8050 fluorometers for specialized workflows: Peltier and circulating-bath temperature controllers for temperature-dependent studies, microvolume cells for limited-sample work, solid-sample holders for films and powders, polarizers for anisotropy measurements, and a microplate reader accessory for 96- and 384-well high-throughput screening. Accessory compatibility varies by model with the fullest accessory compatibility available on FP-8550 and FP-8650.