A fluorometer with optical fiber can be used for a variety of measurements, such as when the sample is larger than the sample chamber, to follow an in-situ reaction, or for measurement in hazardous environments, such as high/low temperature and high pressure. The OBF-832 optical fiber interface is used to connect an optical fiber for remote sampling of fluorescence materials with an FP-8000 Series fluorescence spectrophotometer. By using an optical fiber, measurement can be made by holding the probe close to or immersed in the sample.
In this application note, a comparison was made by measuring samples using an FP-8500 fluorometer with optical fiber and an FLH-809 film holder. To check for spectral accuracy the fiber probe was corrected for quantum efficiency using a calibrated light source with a validated spectral emission.
Measurement System and Condition
|OBF-832||Optical fiber unit|
|Calibration||ESC-842 Calibration light source / standard white reference plate|
|Samples||Red and Yellow reference cards|
Measurement of the Calibrated Light Source under Dark Room Condition
|Excitation Bandwidth||10 nm|
|Emission Bandwidth||10 nm|
|Excitation Wavelength||310 nm|
|Measurement Range||400 - 800 nm|
|Scan Speed||100 nm/min|
|Data Interval||0.5 nm|
|PMT Voltage||350 V (yellow card), 550 V (red card)|
Spectrofluorometer, Optical fiber, Fluorescence spectrum, fluorescence spectrophotometer
Figures 2 and 3 show the measurement results. The profiles of the spectra of each colored sample measured using both the optical fiber and FLH-809 film holder were consistent with each other.
These results demonstrate that the spectral accuracy obtained using both the fiber probe and film holder yield comparable data.
Required Products and Software
- FP-8300/8500/8600/8700 Spectrofluorometer
- OBF-832 Optical Fiber Unit
- ESC-842 Calibrated WI Light Source