Near-infrared fluorescence labels are currently attracting the attention of the scientific community due to their wide range of biological applications. These labels are ideal for biological studies since biomolecules absorb very little in the near-infrared region. NIR fluorescence labels have shown no adverse effects in vivo and are highly stable.
This application note illustrates how the FP-8700 is used to obtain fluorescence spectra and quantum yields of phosphors in the near-infrared spectral region.
|Emission Wavelength||883 nm||Excitation Wavelength||600 nm|
|Emission Bandwidth||10 nm||Excitation Bandwidth||10 nm|
|Excitation Bandwidth||10 nm||Emission Bandwidth||10 nm|
|Scan Speed||500 nm/min||Scan Speed||500 nm/min|
|Response Time||0.5 sec||Response Time||0.5 sec|
|Data Interval||0.2 nm||Data Interval||0.2 nm|
|Sensitivity||600 V||Sensitivity||600 V|
FP-8700, ESC-842 Calibrated WI light source, ILF-890 Integrating sphere, FPA-810 Powder measurement block, PSH-101 Powder cell, Fluorescence, Near-infrared, Quantum yield
The excitation spectra of YVO4:Nd3+ and YVO4:Nd3+, Yb3+ are shown in Figure 1 and the peaks are labeled with their corresponding excited state transitions from the ground state of Nd3+ (4I9/2).
The fluorescence spectra of YVO4:Nd3+ and YVO4:Nd3+, Yb3+ are shown in Figure 2 and the peaks are labeled with their corresponding transition states from the excited states of Nd3+ (4F3/2) and Yb3+ (2F5/2).
The quantum yields of both YVO4:Nd3+ and YVO4:Nd3+, Yb3+ were calculated and shown in Table 1.
Table 1. Quantum yield results.
|Absorbance (%)||External Quantum Yield||Internal Quantum Yield|
This application note uses a FP-8700 to obtain fluorescence measurements in the NIR spectral region. This data is used to characterize near-infrared phosphors, which can later be used as fluorescent labels in biological studies.
Required Products and Software
- FP-8700 Spectrofluorometer
- FPA-810 Powder Sample Cell Block
- PSH-101 Powder Sample Cell
- ESC-842 Calibrated WI Light Source
- ILF-835 100 mm dia. Integrating Sphere
- KBr Plate Sample Holder
- FWQE-880 Quantum Yield Calculation program
The FP-8750 Spectrofluorometer includes a liquid nitrogen PMT detector for applications that require high sensitivity measurements into the near infrared.
Sophisticated optical system offering the ultimate in sensitivity, spectral accuracy, and flexibility for the most challenging materials and biological samples.