Fluorescence Stopped Flow & Automated Titration

For Rapid Kinetics and Titration Experiments

Fluorescence Stopped Flow/Rapid Kinetics

Fluorescence stopped flow
SFS-852 Stopped-Flow System for Rapid Kinetics

The Fluorescence Stopped Flow accessory is used with either the FP-8300 or FP-8500 for measurement of rapid kinetic events by mixing two or more solutions to initiate a fast chemical reaction with very small delay time. The reaction kinetics are then followed by monitoring the change in the fluorescence intensity.

ModelSFS-852SFS-853SFS-854SFS-852TSFS-853TSFS-854T
Number of Syringes234234
Syringe Volume Options10 mL (standard), 1.0, 2.5, 5.0 mL
Mixing Ratio1:1 to 1:20
Dead Time2.9 msec.
Flow Rate5 mL/sec. (10 mL syringe)
Temperature Control System-Heating/Cooling system utilizing Peltier effect
Peltier Heat Radiation-Water-Cooled
Temperature Setting Range-Cell: 10 to 80°C
Syringe: 10 to 60°C
Temperature Accuracy-±0.5°C (cel)
Optional Accessory50 μL, 100 μL, 500 μL delay line

Automated Titration

Fluorescence automatic titration
ATS-826 Autotitrator

The dual syringe autotitrator is used to monitor changes in the fluorescence intensity as a function of pH, chemical denaturant, or exogenous ligands. The syringes can be operated independently to control the titration volumes  and are fitted with a valve for automated refilling and flushing during extended runs. The titration application program in Spectra Manager controls the autotitrator and automatically corrects for concentration during measurement.

ModelATS-826ATS-827
Compatible CellsMicro cell 5 x 5 mm, rectangular cell 10 x 10 mm
Compatible AccessoriesSTR-811, ETC-814STR-812, EHC-813, ETC-815
Number of Syringes2
Syringe Volume Options1.0 mL (standard), 2.5 mL
Injection AccuracyGreater than 99%
Injection Reproducibility Less than 1%
Injection Pitch0.1% of syringe volume