Found 9617 Results / Page 385 of 481
2017 / Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
Recently reported new phenomenon of Aggregation-Induced Raman Optical Activity is demonstrated here for the first time in the pre-resonance conditions… Continue reading Pre-resonance enhancement of exceptional intensity in aggregation-induced Raman optical activity spectra of lutein derivatives
2016 / Molecular Biotechnology
Enzymes isolated from extremophiles often exhibit superior performance and potential industrial applications. There are several advantages performing biocatalysis at elevated… Continue reading Characterization and Low-Resolution Structure of an Extremely Thermostable Esterase of Potential Biotechnological Interest from Pyrococcus furiosus
2017 / Food Chemistry
Bovine β-lactoglobulin (β-Lg) is recognized as a significant milk allergen in several countries. In this study, β-Lg was isolated and… Continue reading Effects of high hydrostatic pressure on the structure and potential allergenicity of the major allergen bovine β-lactoglobulin
2016 / Physical Chemistry Chemical Physics
The ps–μs dynamics of domain-III of human serum albumin (HSA) has been investigated using a new fluorescent marker selectively labeled… Continue reading Elucidation of the local dynamics of domain-III of human serum albumin over the ps–μs time regime using a new fluorescent label
2016 / Crystal Grown & Design
A facile strategy was developed to fabricate structure controllable microcrystals of ferrocene-based metal–organic (Fc-Ala-BCB) materials through evaporation-induced self-assembly. Two distinctive… Continue reading Shape-Controlled Synthesis of Organometallic Microcrystal-Based Hollow Hexagonal Micromotors through Evaporation-Induced Supramolecular Self-Assembly
2016 / Scientific Reports
The protein alpha-synuclein (αS) self-assembles into toxic beta-sheet aggregates in Parkinson’s disease, while it is proposed that αS forms soluble… Continue reading Arachidonic acid mediates the formation of abundant alpha-helical multimers of alpha-synuclein
2016 / Biophysical Chemistry
Misfolded β-sheet-rich protein aggregates termed amyloid, deposit in vivo leading to debilitating diseases such as Alzheimer’s, prion and renal amyloidosis diseases etc.… Continue reading Wild-type hen egg white lysozyme aggregation in vitro can form self-seeding amyloid conformational variants
2016 / European Journal of Organic Chemistry
A covalent organic helical cage with D3 symmetry bearing two trimethylphenyl and six diethynylallene moieties was synthesized. This cage favors… Continue reading Covalent Organic Helical Cages as Sandwich Compound Containers
2016 / Scientific Reports
Sweet proteins are a family of proteins with no structure or sequence homology, able to elicit a sweet sensation in… Continue reading Sweeter and stronger: enhancing sweetness and stability of the single chain monellin MNEI through molecular design
2016 / Protein Science
Synthetic Biology (or Chemical Biology) is a growing field to which the chemical synthesis of proteins, particularly enzymes, makes a… Continue reading Total chemical synthesis, refolding, and crystallographic structure of fully active immunophilin calstabin 2 (FKBP12.6)
2016 / Biochimie
Sulfatases catalyze hydrolysis of sulfate groups. They have a key role in regulating the sulfation states that determine the function… Continue reading The chondroitin sulfate/dermatan sulfate 4-O-endosulfatase from marine bacterium Vibrio sp FC509 is a dimeric species: Biophysical characterization of an endosulfatase
2016 / The Journal of Steroid Biochemistry and Molecular Biology
The cytochrome P450 aromatase is involved in the last step of sex hormones biosynthesis by converting androgens into estrogens. The… Continue reading Impact of R264C and R264H polymorphisms in human aromatase function
2016 / PLoS ONE
SpyTag can spontaneously form a covalent isopeptide bond with its protein partner SpyCatcher. Firefly luciferase from Photinus pyralis was cyclized in vivo by fusing… Continue reading SpyTag can spontaneously form a covalent isopeptide bond with its protein partner SpyCatcher. Firefly luciferase from Photinus pyralis was cyclized in vivo by fusing SpyCatcher at the N terminus and SpyTag at the C terminus. Circular LUC was more thermostable and alkali-tolerant than the wild type, without compromising the specific activity. Structural analysis indicated that the cyclized LUC increased the thermodynamic stability of the structure and remained more properly folded at high temperatures when compared with the wild type. We also prepared an N-terminally and C-terminally shortened form of the SpyCatcher protein and cyclization using this truncated form led to even more thermostability than the original form. Our findings suggest that cyclization with SpyTag and SpyCatcher is a promising and effective strategy to enhance thermostability of enzymes.
2016 / PLoS ONE
In the present work we combine spectroscopic, DSC and computational approaches to examine the multiple extracellular Glu mutants E204Q/E194Q, E204Q/E194Q/E9Q… Continue reading Identification of Specific Effect of Chloride on the Spectral Properties and Structural Stability of Multiple Extracellular Glutamic Acid Mutants of Bacteriorhodopsin
2005 / The Journal of Biological Chemistry
The phospholipase A2 (PLA2) enzymes are activated by binding to phospholipid membranes. Although the N-terminal α-helix of group I/II PLA2s plays… Continue reading Evidence for the Regulatory Role of the N-terminal Helix of Secretory Phospholipase A2 from Studies on Native and Chimeric Proteins
2007 / Journal of Molecular Biology
Cholera toxin (CT) moves from the cell surface to the endoplasmic reticulum (ER) by vesicular transport. In the ER, the… Continue reading Conformational Instability of the Cholera Toxin A1 Polypeptide
2006 / Biochemistry
Phospholipase A2 (PLA2) enzymes become activated by binding to biological membranes and hydrolyze phospholipids to free fatty acids and lyso-phospholipids, the… Continue reading Structural and Functional Effects of Tryptophans Inserted into the Membrane-binding and Substrate-binding Sites of Human Group IIA Phospholipase A2
2016 / Tetrahedron
C2-symmetric binaphthol derivatives bearing two bulky C3-symmetric triphenylsilyl side groups, open-style 3,3′-bis(triphenylsilyl)-1,1′-bi-2-naphthol and closed-style 3,3′-bis(triphenylsilyl)-1,1′-binaphthyl-2,2′-diyl hydrogenphosphate, emit circularly polarized luminescence (CPL)… Continue reading Binaphthyl luminophores with triphenylsilyl groups: sign inversion of circularly polarized luminescence and circular dichroism
2016 / PLoS ONE
Methoprene tolerant protein (Met) has recently been confirmed as the long-sought juvenile hormone (JH) receptor. This protein plays a significant… Continue reading Intrinsic Disorder of the C-Terminal Domain of DrosophilaMethoprene-Tolerant Protein
2016 / Biochimie
The insomniac protein of Drosophila melanogaster (INC) has a crucial role in sleep homeostasis as flies lacking the inc gene exhibit strikingly… Continue reading Proteins involved in sleep homeostasis: biophysical characterization of INC and its partners
