2002 / Archives of Biochemistry and Biophysics
Glycosylated dimeric alliinase (EC 4.4.1.4) was purified to homogeneity from its natural source, garlic. With 660 units/mg, the specific enzymatic activity… Continue reading Purification, characterization, and crystallization of alliinase from garlic
2005 / Nucleic Acids Research
Through binding and fluorescence studies of oligonucleotides covalently attached to a pyrene group via one carbon linker at the sugar… Continue reading Pyrene is highly emissive when attached to the RNA duplex but not to the DNA duplex: the structural basis of this difference
2000 / Journal of Natural Products
Two new lavandulylated flavanones, (2S)-2‘-methoxykurarinone (1) and (−)-kurarinone (2), were isolated from the root of Sophora flavescens, together with two known lavandulyl… Continue reading Cytotoxic Lavandulyl Flavanones from Sophora flavescens
2010 / The Journal of Membrane Biology
Circular dichroism (CD) spectroscopy is an essential tool for determining the conformation of proteins and peptides in membranes. It can… Continue reading CD Spectroscopy of Peptides and Proteins Bound to Large Unilamellar Vesicles
2016 / Protein Engineering, Design & Selection
Structure-based protein design tests our understanding of the minimal determinants of protein structure and function. Previous studies have demonstrated that… Continue reading Probing the minimal determinants of zinc binding with computational protein design
2016 / Dalton Transactions
A combination of mass spectrometry, Raman microspectroscopy, circular dichroism and X-ray crystallography has been used to obtain detailed information on… Continue reading Mapping the protein-binding sites for iridium(III)-based CO-releasing molecules
2016 / Journal of Molecular Structure
In this work the complexation of the guest molecule (dibenzylthiourea-DBT) with α and β forms of hydroxypropylated cyclodextrins was evaluated… Continue reading Inclusion compounds of dibenzylthiourea with hydroxypropylated-cyclodextrins for corrosion protection of carbon steel in acidic medium
2016 / Scientific Reports
In this study, to systematically investigate the targeting specificity of membrane-active peptides on different types of cell membranes, we evaluated… Continue reading Specificity and mechanism of action of alpha-helical membrane-active peptides interacting with model and biological membranes by single-molecule force spectroscopy
2016 / Scientific Reports
Apurinic/apyrimidinic (AP) sites are constantly formed in cellular DNA due to instability of the glycosidic bond, particularly at purines and… Continue reading Catalysts of DNA Strand Cleavage at Apurinic/Apyrimidinic Sites
2016 / RSC Advances
In this article we describe two solid-phase synthetic routes to obtain a nucleo-oligolysine α-peptide containing all four natural nucleobases. The… Continue reading Synthetic approaches to nucleopeptides containing all four nucleobases, and nucleic acid-binding studies on a mixed-sequence nucleo-oligolysine
2016 / Analytica Chimica Acta
A label-free sensing assay for ethanolamine (EA) detection based on G-quadruplex-EA binding interaction is presented by using G-rich aptamer DNA… Continue reading Aptamer-based biosensor for label-free detection of ethanolamine by electrochemical impedance spectroscopy
2016 / Scientific Reports
Proteins, covalently modified by catechol estrogens (CEs), were identified recently from the blood serum of diabetic patients and referred to… Continue reading Site-specific covalent modifications of human insulin by catechol estrogens: Reactivity and induced structural and functional changes
2016 / Scientific Reports
Inorganic phosphate (Pi) is a central signaling molecule that modulates virulence in various pathogens. In Pseudomonas aeruginosa, low Pi concentrations induce… Continue reading Two different mechanisms mediate chemotaxis to inorganic phosphate inPseudomonas aeruginosa
2005 / Cellular and Molecular Life Sciences
We investigated the structural effects induced by Al3+ on different b-amyloid (Ab) fragments at pH 7.4 and T= 25°C, with… Continue reading Aluminum-triggered structural modifications and aggregation of β-amyloids
2003 / PNAS
Observations that beta-sheet proteins form amyloid fibrils under at least partially denaturing conditions has raised questions as to whether these… Continue reading Myoglobin forms amyloid fibrils by association of unfolded polypeptide segments
2003 / Biochemical Pharmacology
The plant derived flavonoid compound quercetin, possesses wide range of biological activities in the human body by interacting with nucleic… Continue reading Probing the binding of the flavonoid, quercetin to human serum albumin by circular dichroism, electronic absorption spectroscopy and molecular modelling methods
2012 / Nature Communications
Bestowing chirality to metals is central in fields such as heterogeneous catalysis and modern optics. Although the bulk phase of… Continue reading First enantioseparation and circular dichroism spectra of Au38 clusters protected by achiral ligands
2004 / Nucleic Acids Research
The interaction of DNA with Mn2+ was studied in absorbance and optical activity in the electronic and vibrational regions. Based… Continue reading The effect of manganese(II) on DNA structure: electronic and vibrational circular dichroism studies
2004 / Nanotechnology
Chiral biomolecules conjugated with silver nanoparticles were investigated by circular dichroism (CD) spectroscopy. Silver nanoparticles were prepared by the citrate… Continue reading Circular dichroism study of chiral biomolecules conjugated with silver nanoparticles
2005 / FEBS Letters
Since it was implicated in a number of neurodegenerative conditions, such as Alzheimer disease, formation of β-sheet-rich protein fibrils (amyloids)… Continue reading Chiral bias of amyloid fibrils revealed by the twisted conformation of Thioflavin T: An induced circular dichroism/DFT study
