Engineering the affinity of a family 11 carbohydrate binding module to improve binding of branched over unbranched polysaccharides

2018 / International Journal of Biological Macromolecules

Carbohydrate binding modules (CBMs) are non-catalytic domains within larger multidomain polypeptides. The CelH from Ruminoclostridium (Clostridium) thermocellum contains a family 11 CBM (RtCBM11) with high binding …

Leah Pandiscia, PhD

In silico modeling of Plasmodium falciparum chloroquine resistance transporter protein and biochemical studies suggest its key contribution to chloroquine resistance

2018 / Acta Tropica

Chloroquine (CQ) has been used for decades as the primary chemotherapeutic drugfor the treatment of malaria. The emergence of drug resistance in Plasmodiumfalciparum has been considered to …

Leah Pandiscia, PhD

Ligand binding properties of two Brugia malayi fatty acid and retinol (FAR) binding proteins and their vaccine efficacies against challenge infection in gerbils

2018 / PLOS Neglected Tropical Diseases

Parasitic nematodes produce an unusual class of fatty acid and retinol (FAR)-binding proteins that may scavenge host fatty acids and …

Leah Pandiscia, PhD

Enzyme Kinetics from Circular Dichroism of Insulin Reveals Mechanistic Insights into the Regulation of Insulin-degrading Enzyme

2018 / Bioscience Reports

Insulin-degrading enzyme (IDE) is a zinc metalloprotease that selectively degrades biologically important substrates associated with type 2 diabetes and Alzheimer’s …

Leah Pandiscia, PhD

Domain architecture of BAF250a reveals the ARID and ARM-repeat domains with implication in function and assembly of the BAF remodeling complex

2018 / PLoS ONE

BAF250a and BAF250b are subunits of the SWI/SNF chromatin-remodeling complex that recruit the complex to chromatin allowing transcriptional activation of …

Heather Haffner

Questions of Mirror Symmetry at the Photoexcited and Ground States of Non-Rigid Luminophores Raised by Circularly Polarized Luminescence and Circular Dichroism Spectroscopy: Part 1. Oligofluorenes, Oligophenylenes, Binaphthyls and Fused Aromatics

2018 / molecules

We report experimental tests of whether non-rigid, π-conjugated luminophores in the photoexcited (S1) and ground (S0) states dissolved in achiral liquids …

Heather Haffner

Insulin adsorption onto zinc oxide nanoparticle mediates conformational rearrangement into amyloid-prone structure with enhanced cytotoxic propensity

2019 / Biochimica et Biophysica Acta (BBA) - General Subjects

Injection localized amyloidosis is one of the most prevalent disorders in type II diabetes mellitus (TIIDM) patients relying on insulin …

Leah Pandiscia, PhD

Recombinant AfusinC, an anionic fungal CSαβ defensin from Aspergillus fumigatus, exhibits antimicrobial activity against gram-positive bacteria

2018 / PLoS ONE

Antimicrobial peptides (AMPs) are short and generally positively charged peptides found in a wide variety of organisms. CSαβ defensins are …

Leah Pandiscia, PhD

Supramolecular organization of perfluorinated 1H-indazoles in the solid state using X-ray crystallography, SSNMR and sensitive (VCD) and non sensitive (MIR, FIR and Raman) to chirality vibrational spectroscopies

2017 / Physical Chemistry Chemical Physics

1H-Indazole derivatives exhibit a remarkable property since some of them form chiral supramolecular structures starting from achiral monomers. The present …

Carlos Morillo

Conformational landscape and hydrogen bonding in (S)-(−)-perillyc acid: experimental VCD, IR, Raman, and theoretical DFT studies

2012 / Tetrahedron: Asymmetry

(S)-(−)-Perillyc acid (4-isopropenylcyclohexene-1-carboxylic acid) is an intermediate in the limonene and pinene pathway degradation and its measurement in urine is …

Carlos Morillo